SubtiBank SubtiBank
yqhY [2017-8-11 16:0:3]
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yqhY [2017-8-11 16:0:3]

modulator of lipid biosynthesis
locus
BSU24330
pI
4.41
mw
14.55 kDa
protein length
135 aa Sequence Blast
gene length
405 bp Sequence Blast
function
control of fatty acid biosynthesis
product
modulator of lipid biosynthesis
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.13|Quasi-essential genes]

    • Gene

    Coordinates
    2,529,926 → 2,530,333

    Phenotypes of a mutant

  • the mutant readily acquires suppressor mutants that result in reduced activity of the [SW|ACCase] [pubmed|28579978]
  • the mutant forms lipophilic clusters [pubmed|28579978]

    • The protein

    Protein family

  • asp23 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|YloU]

    • [SW|Localization]

  • cell poles [pubmed|28579978]

    • Expression and Regulation

    Operons

    genes
    [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|yqhY]
    description
    [Pubmed|7592499]

    regulation

  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]
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    Biological materials

    Mutant

  • MGNA-C367 (yqhY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2365 NBRP B. subtilis, Japan]
  • GP1468 (Δ''yqhY''::''erm''), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]
  • GP1765 (Δ''yqhY''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]

    • Expression vector

  • GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [SW|Jörg Stülke]'s lab
  • pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1471 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1481 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

  • 17114254,7592499,23420519,24178028,28579978